Dose-dependent downregulation of bryo-1–induced IL-8 and IL-1β mRNA by PKC inhibitors. Monocytes were preincubated with the indicated concentrations of staurosporine or UCN-01 for 1 hour and then stimulated with bryo-1 (0.1 ng/mL) for 3 hours. Northern blot analysis was performed as previously described, and the same filter was sequentially hybridized with IL-8 (upper panel), IL-1β (middle panel), and GAPDH (lower panel) probes.