Recombinant AAV-CMV-LacZ vector. LacZ expression cassette was inserted between the ITRs (inverted terminal repeats) of a pUC-based plasmid containing both the AAV ITRs and the AAV rep and cap genes outside the ITRs. Cloning sites used for vector construction are shown. CMV, CMV promoter; An, simian virus 40 (SV40) early polyadenylation signal.