Detection of the HLA-H Cys 282 Tyr mutation by the presence or absence of specific PCR products. The top panel shows the results of the gel pattern digested by Bcg I; the bottom panel was digested by Rsa I. Lane 1 is the uncut control, lanes 2 to 6 are cases of known hemochromatosis, lane 7 is 1 of the 221 β-thalassemia minor cases, lane 8 is 1 of the 280 cases of the general population. M represents the marker.