In situ hybridization analysis of E-selectin and P-selectin mRNA expression in the spinal cord of a SJL/J mouse afflicted with acute EAE (day 14, clinical score +++) is shown. Neither E-selectin nor P-selectin mRNA can be detected. Vessels surrounded by inflammatory cuffs are shown, (a) and (c), dark field; (b) and (d), bright field. Sections are counterstained with toluidine blue. Original magnification × 480.