In situ hybridization analysis of VCAM-1 mRNA expression in the spinal cord of a SJL/J mouse afflicted with acute EAE (day 14, clinical score +++) is shown. A strong hybridization signal for VCAM-1 mRNA can be detected in a vessel surrounded by an inflammatory cuff (A), dark field; (B), bright field. Sections are counterstained with toluidine blue. Original magnification × 480.