E- and P-selectin gene expression is induced in peripheral vessels outside the CNS after injection of LPS or TNF-α. In situ hybridization analysis of E- and P-selectin mRNA expression in the tongue of a SJL/J mouse 4 hours after injection of 50 μg LPS is shown. (a), dark field; (b), bright field; (c), dark field; (d), bright field. Sections are counterstained with toluidine blue. Original magnification × 480.