Induction of E- and P-selectin on primary MBEs in vitro. The same cells are shown from left to right: surface, green fluorescence membrane–staining of live MBEs; vWF, red fluorescence–staining of intracellular vWF in fixed MBEs. E- and P-selectin can be induced on the surface of some vWF-positive MBEs after treatment with LPS (10 μg/mL; 3 hours). No LPS mediated induction of E- and P-selectin when MBEs were cultured in astrocyte conditioned medium (+ ACM) for 5 days. Original magnification × 400.