Fig. 5.
Expression of bcl-x mRNA in HL-60 and CD34+ cells differentiated into monocytes/macrophages. Total RNA was purified from HL-60 cells treated with PMA for 4 days and CD34+ cells after 21 days of culture in the presence of M-CSF, and subjected to RT-PCR analysis with oligonucleotide primers that amplify both bcl-xL (340 bp) and bcl-xS (151 bp). Plasmids containing bcl-xL (SFFV-bcl-xL ) or bcl-xS (SFFV-bcl-xS ) cDNAs were also amplified as positive controls. After 30 cycles, PCR products were electrophoresed in a 2% agarose gel and stained with ethidium bromide.
