(A) Regulation of bcl-2 protein expression in WSU-CLL and I83CLL cells upon treatment with various concentrations of flavopiridol. The cells (1 × 10⁶/mL) were cultured alone (CTRL) or in the presence of flavopiridol (flavo) (200, 400, 800 nmol/L). (B) Exposure of WSU-CLL cells to fludarabine (FAra) (1, 5 μmol/L), genistein (Genist) (50 μmol/L), or quercetin (Querc) (50 μmol/L). Twenty micrograms of protein/lane from cell lysates were loaded on a 12.5% SDS-PAGE gel and electrophoresed. Bcl-2–specific protein was detected using an anti–bcl-2 monoclonal antibody (Dako).