American Society of Hematology

Fig. 3.

$Fig. 3. Apoptosis in HL60, U937, and KG1 cells incubated with ATRA, 1,25(OH)2 D3, and GM-CSF for 72 hours and then exposed to idarubicin 50 μg/L for 24 hours. (A) Apoptosis induced by idarubicin 50 μg/L. Results shown are mean of n = 3 (HL60 & KG1) or n = 7 (U937) experiments; error bars omitted for clarity. Statistical significance: * result statistically different from control; # result statistically different from ATRA/1,25(OH)2 D3 alone, eg, ATRA versus ATRA+GM-CSF; for P values see Table 2. (B) Representative cell-cycle profiles for U937 cells incubated with ATRA and ATRA + GM-CSF, and then exposed to idarubicin 50 μg/L; see Materials and Methods for details: X-axis, red fluorescence, Y-axis, cell number; left-hand plots, after initial incubation (ATRA ± GM-CSF, 72 hours), right-hand plots, after exposure to idarubicin (24 hours); gate B denotes apoptotic fraction, C cells in G0/G1, E S phase, and F G2/M.$

Apoptosis in HL60, U937, and KG1 cells incubated with ATRA, 1,25(OH)₂ D3, and GM-CSF for 72 hours and then exposed to idarubicin 50 μg/L for 24 hours. (A) Apoptosis induced by idarubicin 50 μg/L. Results shown are mean of n = 3 (HL60 & KG1) or n = 7 (U937) experiments; error bars omitted for clarity. Statistical significance: * result statistically different from control; # result statistically different from ATRA/1,25(OH)₂ D3 alone, eg, ATRA versus ATRA+GM-CSF; for P values see Table 2. (B) Representative cell-cycle profiles for U937 cells incubated with ATRA and ATRA + GM-CSF, and then exposed to idarubicin 50 μg/L; see Materials and Methods for details: X-axis, red fluorescence, Y-axis, cell number; left-hand plots, after initial incubation (ATRA ± GM-CSF, 72 hours), right-hand plots, after exposure to idarubicin (24 hours); gate B denotes apoptotic fraction, C cells in G0/G1, E S phase, and F G2/M.

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