Fig. 1.
IEF of natural and recombinant Hbs. Lane 1, natural HbA; lane 2, HbS; lane 3, Hb E6V(β)/E121R(β) double mutant; lane 4, Hb D75Y(α)/E6V(β)/E121R(β) triple mutant; lane 5, D6A(α)/D75Y(α)/E6V(β)/E121R(β); lane 6, HbS. The anode is at the top and the cathode is at the bottom.