Fig. 2.
Separation of α- and β-chains of natural HbS and recombinant HbS double, triple, and quadruple mutants. The samples were applied to a Vydac C4 HPLC column and eluted as described in the text. The amounts of Hb applied were about 100 μg.
Separation of α- and β-chains of natural HbS and recombinant HbS double, triple, and quadruple mutants. The samples were applied to a Vydac C4 HPLC column and eluted as described in the text. The amounts of Hb applied were about 100 μg.
