LasBD does not suppress p190^BCR/ABL expression and does not affect the function of 32D^p190 cells. (A) 32D^p190 cells were transduced with LasBD and selected with MTX and rMuIL-3. Untransduced (in lanes with minus sign) and MTX-selected, transduced cells (in lanes with plus sign) were analyzed by semiquantitative RT-PCR to detect AS mRNA, BCR/ABL mRNA, and β-actin mRNA (left panel) and by Western blot to detect p190^BCR/ABL and p145^ABL protein (right panel), as described in the Materials and Methods. (B) LasBD-transduced (clones A and B) and untransduced 32D^p190 cells were cultured for 6 days in IL-3–containing (left panel) or IL-3–free (right panel) cultures and viable cell expansion was measured.