Effect of pretreatment (on day −14) with IL-12 (200 ng/mouse, IP), IL-10 (1 μg/mouse, IP) or LPS (500 μg/mouse, IP) on the decrease in body weight (A) and food intake (B) induced by IL-12 (400 ng/mouse, IP; once daily for 5 days). The effect of a predose of IFN-γ (1 or 10 μg/mouse, IP), as well as of IL-12 (200 ng) as a reference, is shown in panels C (body weight) and D (food intake). Body weight is expressed as mean ± SD (n = 5). Food intake was evaluated per cage with five mice. (A and B) (□), Vehicle; (♦), IL-12; (•), IL-12/IL-12; (▵), LPS/IL-12; (*), IL-10/IL-12. (C and D) (□), Vehicle; (♦), IL-12; (•), IL-12/IL-12; (▵), IFN-γ 10/IL-12; (*), IFN-γ 1/IL-12.