Fig. 2.
Fig. 2. Tyrosine phosphorylation of cellular proteins in Ba/F3-GMRα cell lines expressing mutant GMRβ. Cells (107) were incubated in the absence or presence of GM-CSF (10 ng/mL) for 15 minutes. Cell lysates were immunoprecipitated with antiphosphotyrosine antibody (A) or anti-β chain antibody (B), electrophoresed through 6% to 12% gradient SDS-PAGE, and then analyzed by immunoblotting with antiphosphotyrosine antibody 4G10.

Tyrosine phosphorylation of cellular proteins in Ba/F3-GMRα cell lines expressing mutant GMRβ. Cells (107) were incubated in the absence or presence of GM-CSF (10 ng/mL) for 15 minutes. Cell lysates were immunoprecipitated with antiphosphotyrosine antibody (A) or anti-β chain antibody (B), electrophoresed through 6% to 12% gradient SDS-PAGE, and then analyzed by immunoblotting with antiphosphotyrosine antibody 4G10.

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