Genomic analysis of mutant fibrinogen Aα-chain gene. (A) Autoradiography of SSCP gel for exon 5 of fibrinogen Aα-chain gene. Cases no. 1 (affected father) and 2 (affected son) presented abnormal migrating bands in addition to the normal ones. DNAs with the known amyloidogenic fibrinogen Aα gene mutations Leu554, Val526 and 4904delG, were analyzed on the same gel for comparison. Unlabeled lanes are normal controls. (B) Autoradiography of DNA sequencing gel of exon 5 of the propositus. The propositus is heterozygous with the normal fibrinogen Aα-chain gene and the mutant gene that is missing T at the second base of codon 522. (C) Ethidium bromide-stained agarose gel of PCR-induced mutation restriction analysis for the detection of the 4897delT mutant gene. Case no. 1, affected father; case no. 2, affected son; controls no. 1 and 2, normal subjects; marker, DNA size marker (ΦX 174 DNA-HaeIII digest). The numbers on the left denote the sizes of the bands in basepairs.