Fig. 5.
Determination of GPO and catalase activity in human hemolysate. The same procedure is applied as shown in Fig 4. (A) Addition of highly diluted hemolysate (9.4 × 10−2 g Hb/L). (B) Inhibition of catalase by 1 mmol/L NaN3 . (C) Second addition of 10−5 mol/L H2O2 . (D) Addition of 2 mmol/L GSH and calibration of the measuring system. (E) Addition of 10−4 mol/L H2O2 . A 10-fold higher concentrated hemolysate was used (for D to E) to better characterize the GPO-mediated decomposition of H2O2 .
