Clonogenic growth of Kit expressing MTHC. Cells from the same cultures of parental, WTKit, and V816Kit MTHC as for Fig 2, were plated at 3,000 per dish in methylcellulose containing murine GM-CSF, human SCF, or no factor. The graphs show the average number of colonies ± standard error of mean (SEM) at 2 weeks after plating. (A) Shows the results for nonadherent cells collected from suspension and (B) the results for adherent cells from the same culture, harvested by trypsinization.