Fig. 7.
Phagocytosis of fluorescent beads by adherent MTHC. The images in the two columns are of the same field of view as observed by phase-contrast microscopy shown on the left (A through C) and fluorescence microscopy shown on the right (D through F ). The cells can be seen in the images on the left and the phagocytosed fluorescent beads on the right. The number of the cells in the field of view is indicative of the proportion of cells that were strongly adherent to glass in cultures of similar cell density. (A and D) Show parental MTHC, (B and E) show WTKit-expressing MTHC, and (C and F ) show V816Kit-expressing MTHC. The cells were observed at an initial magnification of 40×.