The RT-PCR expression of TGF-β, GM-CSFRα, and G3PDH. (A) RT-PCR of TGF-β in samples of complete tonsil, germinal center (GC), and extra-germinal center (EGC), showing a single-positive band (161 bp). Lane 1, 100-bp ladder; lane 2, total tonsil (case 1); lane 3, total tonsil except reverse transcriptase (patient 1); lanes 4 and 5, total tonsil (patients 2 and 3); lane 6, GC (patient 4); lane 7, EGC (patient 4); lane 8, primer confirmed as a positive control. (B) RT-PCR of GM-CSFRα in samples of total tonsil and GC, showing a single-positive band (682 bp). Lane 1, 100-bp ladder; lane 2, total tonsil (patient 1); lane 3, total tonsil except reverse transcriptase (patient 1); lanes 4 and 5, total tonsil (patients 2 and 3); lane 6, GC (patient 4); lane 7, EGC (patient 4); lane 8, primer confirmed as a positive control. (C) RT-PCR of G3PDH in samples of total tonsil, GC, and EGC, showing a single-positive band (983 bp). Lane 1, 100-bp ladder; lane 2, total tonsil (patient 1); lanes 3 and 4, total tonsil (patients 2 and 3); lane 5, GC (patient 4); lane 6, EGC (patient 4); lane 7, primer confirmed as a positive control.