Fig. 2.
Cytofluorometric analysis of vWF–A1-domain expressed on COS-7 cells. Transfected cells were first incubated with the monoclonal mouse antibodies anti-FLAG (A and B), LJ-RG-46, specifically detecting human A1-domain (C), and anti–c-myc (D) as a nonspecific control followed by incubation with a FITC-conjugated anti-mouse antibody for 20 minutes at 4°C. At least 20,000 cells were counted each experiment.
