Western blot analysis of COS-7 cell–expressed human and porcine vWF–A₁-domain constructs under reducing (R) and nonreducing (NR) conditions. Preparations of COS-7 cells transfected with standard porcine (P_473-713) and human (H_473-713) constructs or a further truncated human (aa 475-709: H_475-709) vWF-A₁ construct as well as with empty vector (E) are shown. (A) Detecting antibody LJ-RG46 (mouse anti-human A1-domain MoAb). (B) Detecting antibody α-FLAG (mouse anti-FLAG MoAb).