Fig. 5.
![Fig. 5. Platelet aggregation and Ca++ influx. Washed platelets were prepared as described under Materials and Methods. For intracellular Ca++ uptake (Δ[Ca2+]i) detection, platelets were loaded with the photoprotein aequorin. MgCl2 (1.0 mmol/L), CaCl2 (1.0 mmol/L), and fibrinogen (final concentration: 800 μg/mL) were added before the experiments. Aggregation and luminescence following incubation with transfected COS-7 cells were recorded simultaneously. Each box displays one representative experiment for COS-7 to platelet ratios of 1:280. The upper (+)-tagged lines show human platelet aggregation as percentage of light transmission. The lower (×)-tagged lines give the simultaneously recorded change in luminescence due to platelet Δ[Ca2+]i .](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/11/10.1182_blood.v90.11.4425/3/bl_0013f5.jpeg?Expires=1735505260&Signature=0Ut66YU-VdTuPfc3IksNlEMS3vAF2i~be6Uq9OBE6OL9EVT2pGZxZqzqJ4Onv5d19S6j0SmWTppZ6OrxPwNKs8k3DYktWvNUefTb9I4w~AwIiACzIZ3OFMH0n~3O-x6cqNmsSlf-9hr7NVBCc8U9zC21clUBXSUHiOnu7AXTJINsPPV1WIeiodnuOFE02WOu21Pp0WkCQ1tCUrWwXutC7I3wh-s-aXMDXaeaf1oRNUZc8VEGa5ynF-SeOI6KBQkdLbb~Bjp3cB7Aa7KDFTlLv9S1PZEy3SreTYuKukUDloeWxBGqIcsmtjrL0D7rLgaUqNJeFxTG81yti8GJzhxY4g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Platelet aggregation and Ca++ influx. Washed platelets were prepared as described under Materials and Methods. For intracellular Ca++ uptake (Δ[Ca2+]i) detection, platelets were loaded with the photoprotein aequorin. MgCl2 (1.0 mmol/L), CaCl2 (1.0 mmol/L), and fibrinogen (final concentration: 800 μg/mL) were added before the experiments. Aggregation and luminescence following incubation with transfected COS-7 cells were recorded simultaneously. Each box displays one representative experiment for COS-7 to platelet ratios of 1:280. The upper (+)-tagged lines show human platelet aggregation as percentage of light transmission. The lower (×)-tagged lines give the simultaneously recorded change in luminescence due to platelet Δ[Ca2+]i .
