Fig. 3.
Coexpression of Bmx induces activation of Stat5. (A) COS cells were transfected with Stat5 expression vector alone or together with Bmx-HA expression vector. Cell lysates were electrophoresed in 7.5% SDS-PAGE and Western immunoblotted with anti-Stat5 and anti-phosphotyrosine antibodies. (B) The same lysates were analyzed in a mobility shift assay using 32P-labeled oligonucleotide from the promoter of the β-casein gene. The lysates were also supershifted with anti-Stat5 antibody.
