Relationship between log of reciprocal delay time and Hb concentration for polymerization of Hb F γG1V, γE5P, γS143H/Hb S mixtures. Kinetics of polymerization of the deoxy-forms of 1:1 mixtures of Hb F γG1V, γE5P, γS143H/Hb S compared with Hb F/Hb S or Hb A₂ /Hb S mixtures were performed in 1.8 mol/L phosphate buffer, pH 7.4, at 30°C using the temperature-jump method. ▵, ○, and X represent Hb F γG1V, γE5P, γS143H/S, F/S, and A₂ /S mixtures, respectively, whereas • represents HbS.