American Society of Hematology

Fig. 3.

$Fig. 3. Selective retention of natural IgG autoantibody activity by IVIgM coupled to Sepharose. IVIg (50 mg) was subjected to affinity chromatography on 60 mg of IVIgM coupled to Sepharose. The acid-eluted fraction of the column (▪) and the loaded material (□) were then compared for reactivity with a panel of self (TG, MG, and TF ) and non-self (PLD and PK) antigens by ELISA.$

Selective retention of natural IgG autoantibody activity by IVIgM coupled to Sepharose. IVIg (50 mg) was subjected to affinity chromatography on 60 mg of IVIgM coupled to Sepharose. The acid-eluted fraction of the column (▪) and the loaded material (□) were then compared for reactivity with a panel of self (TG, MG, and TF ) and non-self (PLD and PK) antigens by ELISA.

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