Fig. 5.
Competitive binding of IVIgM and heterologous anti-idiotypes to idiotypes of anti-F.VIII autoantibodies. Microtiter plates were coated with F(ab′)2 fragments of IgG purified from the plasma of a patient with anti-F.VIII autoimmune disease and incubated with 0.025 μg of anti-idiotypic monoclonal antibody 20F2 in the presence of increasing concentrations of IVIgM (▵), monoclonal IgM (•), or IVIg (▪). Bound anti-idiotype was then measured using peroxidase-labeled antimouse Ig antibodies. The abscissa represents the molar ratio between competitor Ig and monoclonal antibody 20F2.
