Fig. 6.
Competitive binding of IVIgM and heterologous anti-idiotypes to idiotypes of anti-TG autoantibodies. Microtiter plates were coated with F(ab′)2 fragments of IgG expressing the T44, C2, and 1B4 idiotypes; purified from serum of a patient with Hashimoto's thyroiditis; and incubated with 0.1 μg of rabbit anti-T44 IgG (A) or with 0.1 μg of anti-idiotypic monoclonal antibodies 1B4 or 1C2 (B and C, respectively) in the presence of increasing concentrations of IVIgM (▵), monoclonal IgM (•), or IVIg (▪). Bound anti-idiotype was then measured using peroxidase-labeled antirabbit or antimouse Ig antibodies. The abscissa represents the molar ratio between competitor Ig and anti-idiotype.
