Fig. 2.
![Fig. 2. Impaired comitogenic effects of solid phase-immobilized fibronectin (FN), anti-CD29 MoAb (4B4), or anti-CD49d MoAb (3G6) on CD3-dependent T-cell proliferation after allo-BMT. Purified lymphocytes obtained from patients after T-cell–depleted allo-BMT (<4 mo, n = 4; 4-12 mo, n = 5; <12 mo, n = 5), and healthy volunteers (n = 20) were stimulated in a serum-free medium by immobilized anti-CD3 MoAb (0.1 μg/mL) with FN (5 μg/mL) or MoAbs (5 μg/mL) against CD2, CD29, and CD49d. After 3-day culture, proliferative response was assessed by [3H]thymidine incorporation. Results are expressed as the mean cpm of triplicate samples and SEM.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/90/10/10.1182_blood.v90.10.4222/3/bl_0030f2.jpeg?Expires=1735595381&Signature=ltDYiSrHGfjjKLtMTY0IhygGdnm4t034y0zQjTL0DGKMUX2kb7Lrc~xXMo1XsBk2SEnNwQnx9Fuz7KapSZ77pOvVNsfYJqnWfP7ZFID0e-DmF5F4jKf0fSbHT-alPq3NXNLUQnj7mUu4MaUvj3FS-e1cZmLU0G0Wtz~qKhv61iqQS-fqIZCO8mCMKrToHqbZwdialnCrYFWOQ4D79KdTxYSyjGhu0scMjpLTUhtL-aaRNCrQ2KiyYMVj6geaB9fuXkHIp2AGtaBFucHsrIENt-t-l-h3X9yDrAJz4ZxYwK7utYPez85X-pyqd~5fiYwo68wuAxAnsJ7xEA8aLeI2Ug__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Impaired comitogenic effects of solid phase-immobilized fibronectin (FN), anti-CD29 MoAb (4B4), or anti-CD49d MoAb (3G6) on CD3-dependent T-cell proliferation after allo-BMT. Purified lymphocytes obtained from patients after T-cell–depleted allo-BMT (<4 mo, n = 4; 4-12 mo, n = 5; <12 mo, n = 5), and healthy volunteers (n = 20) were stimulated in a serum-free medium by immobilized anti-CD3 MoAb (0.1 μg/mL) with FN (5 μg/mL) or MoAbs (5 μg/mL) against CD2, CD29, and CD49d. After 3-day culture, proliferative response was assessed by [3H]thymidine incorporation. Results are expressed as the mean cpm of triplicate samples and SEM.
