Endogenous expression of pp125^FAK, Cas-L, paxillin, Lck, and vinculin in the whole extracts of thymocytes, post-BMT patients' and healthy volunteers' lymphocytes. (A) Whole thymocytes and peripheral T lymphocytes from post-BMT patients or a normal control (50 μg/lane) were analyzed using anti-FAK MoAb. The same membrane was rehybridized with anti-Cas MoAb followed by anti-paxillin MoAb. (B) Immunodepleted cell population from normal thymocytes using anti-CD3 (OKT3) plus anti-CD4 (19Thy) plus anti-CD8 (21Thy) or anti-CD4 plus anti-CD8 MoAbs, whole thymocytes, and peripheral T lymphocytes from post-BMT patients or normal controls were lysed. Each cell lysate (50 μg/lane) was subjected to Western blotting with anti-Lck MoAb followed by anti-vinculin MoAb.