Fig. 2.
Transfection of wild-type MR promoter constructs into myeloid and nonmyeloid cells. Serial 100-bp deletion constructs of the wild-type MR receptor fused to a luciferase reporter gene were transfected into myeloid (U937 and RAW264) and nonmyeloid cells (HeLa and Jurkat) and assayed for activity. Promoter activity (luc/GH) was measured in millivolts (mV) and normalized to 1 μg of growth hormone produced by a cotransfected tk-GH plasmid per milliliter of medium (see the Materials and Methods). Values of the transfected vector backbone (pGLO) are included. Bars represent the average value obtained from between three and six transfections performed on each construct.