American Society of Hematology

Fig. 2.

$Fig. 2. Western blot analysis for GCRER, Δ(5-195)GCRER, Δ(5-195,324-597,702-812)GCRER, and GCR expression in the transfected Ba/F3 cells. Cell lysates of parent Ba/F3 cells (lanes 1 and 5) and the Ba/F3 cells transfected with pCMX-GCRER (lanes 2 and 6), pCMX-Δ(5-195)GCRER (lanes 3 and 7), pCMX-Δ(5-195,324-597,702-812)GCRER (lane 8), and pCMX-GCR (lane 4) were size-fractionated in SDS polyacrylamide gels, and electroblotted to PVDF membranes. Chimeric molecules were detected using anti–G-CSFR antibody (lanes 1, 2, 3, and 4) or anti-ER antibody (lanes 5, 6, 7, and 8). The bands were visualized using alkaline phosphatase–conjugated second antibody and 5-bromo-4-chloro-3-indoyl-phosphate (BCIP)/NBT color development substrate. *Transgene products.$

Western blot analysis for GCRER, Δ(5-195)GCRER, Δ(5-195,324-597,702-812)GCRER, and GCR expression in the transfected Ba/F3 cells. Cell lysates of parent Ba/F3 cells (lanes 1 and 5) and the Ba/F3 cells transfected with pCMX-GCRER (lanes 2 and 6), pCMX-Δ(5-195)GCRER (lanes 3 and 7), pCMX-Δ(5-195,324-597,702-812)GCRER (lane 8), and pCMX-GCR (lane 4) were size-fractionated in SDS polyacrylamide gels, and electroblotted to PVDF membranes. Chimeric molecules were detected using anti–G-CSFR antibody (lanes 1, 2, 3, and 4) or anti-ER antibody (lanes 5, 6, 7, and 8). The bands were visualized using alkaline phosphatase–conjugated second antibody and 5-bromo-4-chloro-3-indoyl-phosphate (BCIP)/NBT color development substrate. *Transgene products.

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