(A) Binding of Bio-GAS and mouse MoAbs CSLEX-1 (anti-sLe^x) and 19.9 (anti-sLe^a) to mock-treated (NIL) and sialidase-treated (NASE) colon carcinoma cell line COLO205. (B and C) Inhibition studies of the binding of Bio-GAS to HL-60 cells by unconjugated CAGAS (Cold IgMGAS), unconjugated IgMPoly (Cold IgMPoly), sialyllactose, lactose, sialic acid, and sucrose. The immunofluorescence technique was as described in Fig 1. Solid and open curves in (A) correspond, respectively, to antibodies and negative controls. In (B), MFI indicates mean fluorescence intensity as obtained by flow cytometry. The MFI value of the 100% CAGAS binding in (C) corresponded to 2,450.