ELISA plate-bound CAGAS captured cancer-associated serum components reactive with anti-sLe^x (CSLEX-1 and KM-93) and anti-sLe^a (19.9 and 2D3) murine MoAbs as well as with rat MoAb HECA-452 that recognize a common epitope in both sLe^x and sLe^a.20,21 30 The results with serial dilutions of 2 (885 and 842) of 12 cancer sera and with 7 (N44-N50) of 14 normal sera (1/10 dilution) are shown. Plate-bound CAGAS did not capture serum components reactive with MoAb CD55 (143-30, of IgG1 isotype such as 19.9) plus MoAb CD8 (109-2D4, of IgM isotype such as CSLEX-1, KM-93, and 2D3). Normal rat serum (1/100 dilution) used in place of HECA-452 showed values not different from those shown for CD55 + CD8 murine MoAbs. The results with plate-bound MPoly or plate-bound anti-i CABER (not shown) were not different from those shown for plate-bound CAGAS with CD55 + CD8 MoAbs. Y-axis and X-axis show, respectively, background-subtracted OD and reciprocal values of dilutions.