(A) ELISA plate-bound CAGAS captured components bearing sLe^x and sLe^a determinants in the supernatant of COLO205 cells, but not in the culture supernatant of HL-60; and a considerable proportion of these components remained soluble in 0.6 N percloric acid (COLO-PCA). The results shown with MoAbs KM-93 and 2D3 were similar to those shown for MoAbs CSLEX-1 and 19.9, respectively. (B) Inhibition by sialyllactose of the binding of plate-bound CAGAS to sLe^x- and sLe^a-bearing components present in COLO205 supernatant (1/8 dilution). (C) Sephacryl S-300 column chromatography of CAGAS-captured components bearing sLe^x and sLe^a present in COLO205 culture supernatant. The void volume (Vo) and the elution peaks of human IgM (19S) and IgG (7S) are indicated. The large peak at OD 280 nm corresponds to the fetal calf serum albumin (FCA) present in the culture medium. The staining of elution fraction Fr1 in the PAS reaction was analyzed using FCA as negative control.