FISH analyses of KMS-11 cell line. (A) Partial metaphase hybridized with a FGFR3 cDNA clone HE8.31 The signals (red) are evident at the 4p16.3 telomeric region of a shorter chromosome 4 (4s) and at the telomeric region of chromosome 14q, der(14), while absent in the normal chromosome 4 homolog, der(4). (B) Partial karyotype from a metaphase hybridized with phage λAB3 (see Fig 2). Signals (red) were found on the shorter chromosome 4 (4s), on der(4), and on der(14), indicating that the 4p16.3 breakpoint occurred within the genomic region represented by this clone.