Expression analysis of the FGFR3 gene. (A) Northern blot analysis of human leukemic cell lines. The length of the FGFR3 transcript is indicated in kb. Ethidium bromide staining is shown below for loading quantification. (B) RT-PCR analysis of FGFR3 expression in MM biopsies (LB375,LB1017, LB411), cell lines (U266, KMS-11), and bone marrow from a normal donor (Normal). The length of the FGFR3 amplified fragment is shown in base pairs. The hybridization with an internal fragment obtained by nested PCR is shown below. The amplification of specific sequences of the β-actin gene is also shown as template control.