Fig. 3.
Detection of CD28RC in EMSA. The nuclear extract from CD4 cells derived from OKT3-stimulated preG-PBMCs was incubated with a32P-labeled CD28RE. Extracts were electrophoresed on a 6% polyacrylamide gel and complexes of nuclear proteins and CD28RE were visualized using ImageQuant3.3 software (Molecular Dynamics). Unlabeled CD28RE (10 and 50 pmol) as a specific competitor or negative regulatory element A (NRE-A) as a nonspecific competitor were added as indicated.
