American Society of Hematology

Fig. 4.

$Fig. 4. EMSA of CD28RC in OKT3-stimulated preG- and G-CD4 cells. 1 × 106/mL unfractionated preG-PBMCs and G-PBMCs were cultured for 4 hours in OKT3-coated T75 culture flasks in the presence or absence of CTLA4Ig. One microgram of nuclear extract isolated from CD4 cells purified by immunomagnetic enrichment was used for the EMSA. Results from one of four experiments are shown.$

EMSA of CD28RC in OKT3-stimulated preG- and G-CD4 cells. 1 × 10⁶/mL unfractionated preG-PBMCs and G-PBMCs were cultured for 4 hours in OKT3-coated T75 culture flasks in the presence or absence of CTLA4Ig. One microgram of nuclear extract isolated from CD4 cells purified by immunomagnetic enrichment was used for the EMSA. Results from one of four experiments are shown.

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