Expression of hematopoietic genes in differentiating control and scl-null EBs. RT-PCR amplification of three fivefold serial dilutions of cDNA for each of the indicated genes is shown for each line at day 0, 4, 6, and 8 of embryoid body differentiation. As in Fig 1, the genotype of the cell line (+/+, +/−, or −/−) is indicated above the panel. Note the discordance in expression between control cells and scl-null cells for GATA-1, EKLF, NF-E2, c-myb, vav, and α globin at the day-8 timepoint.