American Society of Hematology

Fig. 2.

$Fig. 2. The density distribution of all RBCs, F+ cells, T+ cells, T+F+ cells, and T+F− cells. Sickle cells were separated by density and, after permeabilization, each fraction was analyzed for HbF and TfR by two-parameter flow cytometry. An arbitrary total of 10,000 cells was selected for analysis, and the number of each subtype (T+F+ and T+F−) in each density fraction was calculated from the percentage of total cells in each fraction and the percentage of cells in each fraction that belonged to that subtype. Subtype percentages were corrected by subtracting the corresponding percentage in the HbF/idiotype control. The data for total F+ cells was calculated using an average of the idiotype control and specific antibody values. (A) Cells from a patient with a higher number of F+ cells. (B) Cells from a patient with a lower number of F+ cells.$

The density distribution of all RBCs, F⁺ cells, T⁺ cells, T⁺F⁺ cells, and T⁺F⁻ cells. Sickle cells were separated by density and, after permeabilization, each fraction was analyzed for HbF and TfR by two-parameter flow cytometry. An arbitrary total of 10,000 cells was selected for analysis, and the number of each subtype (T⁺F⁺ and T⁺F⁻) in each density fraction was calculated from the percentage of total cells in each fraction and the percentage of cells in each fraction that belonged to that subtype. Subtype percentages were corrected by subtracting the corresponding percentage in the HbF/idiotype control. The data for total F⁺ cells was calculated using an average of the idiotype control and specific antibody values. (A) Cells from a patient with a higher number of F⁺ cells. (B) Cells from a patient with a lower number of F⁺ cells.

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