Lack of activation of the complement system by the mRh1. (A) Lack of complement-dependent lysis of RBCs sensitized by using mRh1. (a) mRh1-sensitized RBCs. (b) Control RBCs. (B) Positive control. (c) RBCs sensitized using mRh1 and an anti-MYC Tag and antimouse Ig enhancing system. (d) Control RBCs lacking mRh1 incubation step. (c′ and d′) Immunofluorescence controls of mRh1 and enhancing system fixation. Scale is expressed in percentage as a ratio of the sample mean fluorescence channel to that of an antiglycophorin A MoAb positive control. Hemolysis is quantified as percentage of the maximal lysis of the system when using a complement fixating antiglycophorin A MoAb.