American Society of Hematology

Fig. 1.

$Fig. 1. RNA with homology to HERV-K10; bands on a sucrose gradient at the typical density of retroviral particles. (A) shows the density profile of a sucrose gradient fractionation of a platelet lysate made from patient UPN07. The arrow indicates the fraction that gave the positive signal shown also arrowed in (B). (B) shows the result of RT-PCR with degenerate oligonucleotide primers. Lanes are (from left to right) M, DNA molecular weight marker; *, no cDNA synthesis step control for DNA template contamination; −, water control; 4, 8, 12, 16, 20, 24, and 28, sucrose fraction numbers; plus (+RT) or minus (−RT) reverse transcriptase.$

RNA with homology to HERV-K10; bands on a sucrose gradient at the typical density of retroviral particles. (A) shows the density profile of a sucrose gradient fractionation of a platelet lysate made from patient UPN07. The arrow indicates the fraction that gave the positive signal shown also arrowed in (B). (B) shows the result of RT-PCR with degenerate oligonucleotide primers. Lanes are (from left to right) M, DNA molecular weight marker; *, no cDNA synthesis step control for DNA template contamination; −, water control; 4, 8, 12, 16, 20, 24, and 28, sucrose fraction numbers; plus (+RT) or minus (−RT) reverse transcriptase.

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