Western blot analysis of RhD proteins from red blood cells of different D^VI and common Rh phenotypes. Total membrane proteins from red blood cells of different D^VI (D^VI ccEe and D^VI Ccee) and common (dccee and DccEE) Rh phenotypes were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12.5%) in nonreducing conditions, transferred to a nitrocellulose membrane (0.45 mm; Schleicher & Schuell, Dassel, Germany), and immunostained with LOR-15C9 monoclonal antibody.8 Immunoblots were finally stained with antihuman IgG peroxidase-tagged antibodies (Biosys, Compiegne, France) and the peroxidase activity was shown by the ECL chemoluminescence system from Amersham (Bucks, UK).