Time-dependent conversion of α dimerization site recombinant peptide monomers into self-aggregates. Recombinant peptide samples were concentrated with a 30K centriprep concentrator to a final concentration of 0.5 mg/mL and 50 μg of protein was immediately analyzed by HPLC gel filtration. The area of each monomer peak on day 0 was set equal to 100%. In parallel experiments, equal amounts of each α spectrin recombinant peptide was injected at intervals over the next 10 days and the amount of monomer in each sample was calculated relative to the amount of monomer observed for that protein at day 0. (□) α18-21; (▵) α18-21¹⁸⁵⁷; (•) α18-21^1857-Δ46.