FXII and TTR production in control and rIL-6–treated HepG2 cells cultured in the presence or in the absence of 10% FCS. HepG2 cells were plated at a density of 1 × 105 cells/cm2 in 25-cm2 flasks. After 24 hours, the medium was replaced with fresh medium with or without 10% (vol/vol) FCS with or without rIL-6 (0 time). After 24 hours of culture, media were harvested and replaced with fresh medium (±rIL-6) and the cells were cultured for an additional 24 hours. FXII (A) and TTR (B) concentrations in the culture media were determined by ELISA. Results were corrected for cell number by assessing the total amount of RNA extracted from the flasks and are expressed as the amount of protein secreted in the culture medium in 24 hours by 106 HepG2 cells. Results (mean ± SD) are the average of three independent experiments in duplicate. * P < .05 as compared with corresponding control.
