Fig. 5.
STA2-induced inactivation of myosin phosphatase in intact platelets. Washed platelets (1 × 106/μL platelets) were stimulated with 1 μmol/L STA2 without stirring. MBS was immunoprecipitated with anti-MBS antibody and the activity of myosin phosphatase was determined immediately as described in the Materials and Methods. Data represent the means ± SE of five independent experiments. Immunoprecipitates were immunoblotted with antibodies against MBS and the PP1δ catalytic subunit (inset).
