Fig. 1.
Cb2 and Cb1 mRNA expression in murine organ tissues. (A) Schematic representation of murine Cb2 and Cb1 mRNA (noncoding exon 1 and protein coding exon 2) and the cDNA probes used for RNAse protection experiments. The shaded boxes represent open reading frames. H, HincII; B, BamHI; S, Stu I; N, Nco I; (E), EcoRI in vector. (B) RNAse protection on 10 μg of total RNA of different mouse organs using Cb2 probe I and Cb1 probe I (see [A]). The protected fragments were 185 bp (Cb2) and 460 bp (Cb1). Sp, spleen; Th, thymus; H, heart; S, stomach; G, gut; K, kidney; B, bladder; L, liver; Br, brain; T, testis.