Fig. 8.
Effects of anandamide and other cannabinoid ligands on G-CSF–induced thymidine incorporation of Cb2-transfected 32D/G-CSF-R cells. (A) 32D/G-CSF-R were transfected with Cb-2 cDNA (32D/G-CSF-R/Cb2) or with pBabe control vector (32D/G-CSF-R/pB). Cells were cultured without G-CSF with different concentrations anandamide ([○] 32D/G-CSF-R/pB; [•] 32D/G-CSF-R/Cb2 ) or with G-CSF plus anandamide ([□] 32D/G-CSF-R/pB; [▪] 32D/G-CSF-R/Cb2). (B) 32D/G-CSF-R-Cb2 cells were cultured with G-CSF plus different concentrations of the distinct cannabinoid ligands, anandamide (▪), WIN 55212-2 (○), Δ8-THC (□), Cannabinol (•), Cannabidiol (▵), and CP55,940 (⋄).