Kinetics of MO7e cell migration, diffusion of SLF, and survival/proliferation effects of SDF-1 and SLF. (A) MO7e cell migration was monitored during 24 hours at indicated time points after setting initial positive gradients with SDF-1 (100 ng/mL) and SLF (10 ng/mL). Viable cell numbers in the lower chambers were counted and the average and range of duplicate were shown. (B) Diffusion of SLF from the lower chamber to the upper chamber was monitored at indicated time points. SLF (10 ng/mL) was added to the lower chambers of the Transwell system to form a positive gradient. (C) MO7e cells were incubated in 24-well plates containing SLF (10 ng/mL), SDF-1 (100 ng/mL), or medium. At indicated time points, viable MO7e cells were counted (see Materials and Methods for details).